Conditioning of Leverage Scores and Computation by QR Decomposition

The leverage scores of a full-column rank matrix A are the squared row norms of any orthonormal basis for range(A). We show that corresponding leverage scores of two matrices A and A + \Delta A are close in the relative sense, if they have large magnitude and if all principal angles between the column spaces of A and A + \Delta A are small. We also show three classes of bounds that are based on perturbation results of QR decompositions. They demonstrate that relative differences between individual leverage scores strongly depend on the particular type of perturbation \Delta A. The bounds imply that the relative accuracy of an individual leverage score depends on: its magnitude and the two-norm condition of A, if \Delta A is a general perturbation; the two-norm condition number of A, if \Delta A is a perturbation with the same norm-wise row-scaling as A; (to first order) neither condition number nor leverage score magnitude, if \Delta A is a component-wise row-scaled perturbation. Numerical experiments confirm the qualitative and quantitative accuracy of our bounds.Comment: This version has been accepted to SIMAX but has not yet gone through copy editin

Effects of light and temperature on germination of Pyxidanthera brevifolia Wells (Diapensiaceae)

Pyxidanthera brevifolia is an evergreen semiwoody cushion plant endemic to the Sandhills of North and South Carolina, with the majority of populations occurring on Fort Bragg Military Reservation in North Carolina. Currently the species is listed as Endangered in North Carolina and is designated as a Species at Risk (SAR) by the US Department of Defense. Previous studies have suggested that seeds may not be viable because they failed to germinate under controlled conditions. Our objectives in this study were to attempt germination of Pyxidanthera brevifolia seeds, determine the best temperature conditions for germination, and understand more about germination requirements to aid in future restoration efforts. Using seeds that had been stored at room temperature for six months, we performed a germination experiment at the NCSU Phytotron with six treatments, all combinations of three temperature regimes (low (18 uC day / 14 uC night), medium (22/18 uC), and high (26/ 22 uC)) and two light conditions (light and dark). We monitored the experiment for 13 weeks, recording the number of seeds germinating per dish and the number of days to germination for seeds in each treatment. We found that Pxyidanthera brevifolia produces germinable seeds and that there are significant effects of light and temperature on germination. Highest germination occurred under low temperature and high light conditions (78%); the combination of high temperature and no light produced the lowest germination (6%). Seeds exposed to light germinated significantly earlier at the coolest temperature, compared to medium and high temperatures. These results indicate that it is possible to germinate seeds of this rare plant and suggest that germination of Pyxidanthera brevifolia likely occurs in late fall and is dependent on adequate light availability

Cosmic Dust Catalog

Since May 1981, the National Aeronautics and Space Administration (NASA) has used aircraft to collect cosmic dust (CD) particles from Earth's stratosphere. Specially designed dust collectors are prepared for flight and processed after flight in an ultraclean (Class-100) laboratory constructed for this purpose at the Lyndon B. Johnson Space Center (JSC) in Houston, Texas. Particles are individually retrieved from the collectors, examined and cataloged, and then made available to the scientific community for research. Cosmic dust thereby joins lunar samples and meteorites as an additional source of extraterrestrial materials for scientific study. This catalog summarizes preliminary observations on 468 particles retrieved from collection surfaces L2021 and L2036. These surfaces were flat plate Large Area Collectors (with a 300 cm2 surface area each) which was coated with silicone oil (dimethyl siloxane) and then flown aboard a NASA ER-2 aircraft during a series of flights that were made during January and February of 1994 (L2021) and June 7 through July 5 of 1994 (L2036). Collector L2021 was flown across the entire southern margin of the US (California to Florida), and collector L2036 was flown from California to Wallops Island, VA and on to New England. These collectors were installed in a specially constructed wing pylon which ensured that the necessary level of cleanliness was maintained between periods of active sampling. During successive periods of high altitude (20 km) cruise, the collectors were exposed in the stratosphere by barometric controls and then retracted into sealed storage container-s prior to descent. In this manner, a total of 35.8 hours of stratospheric exposure was accumulated for collector L2021, and 26 hours for collector L2036

Observation and analysis of in situ carbonaceous matter in Nakhla: Part I

New analyses of indigenous secondary material in the martian meteorite Nakhla reveal amorphous carbon-rich veins and dendrites. The texture and chemistry of this material resembles that of biogenically altered sub-ocean basaltic glasses

Observation and analysis of in situ carbonaceous matter in Nakhla: part II

Analysis of in situ carbonaceous matter in the Nakhla SNC meteorite has been carried out using a variety of techniques. Laser raman data shows the carbonaceous matter to be highly complex and static mass spectrometry has shown it to have an isotopic composition of '18 to '20' C

Evolution and rapid spread of a reassortant A(H3N2) virus that predominated the 2017-2018 influenza season

The 2017-2018 North American influenza season caused more hospitalizations and deaths than any year since the 2009 H1N1 pandemic. The majority of recorded influenza infections were caused by A(H3N2) viruses, with most of the virus's North American diversity falling into the A2 clade. Within A2, we observe a subclade which we call A2/re that rose to comprise almost 70 per cent of A(H3N2) viruses circulating in North America by early 2018. Unlike most fast-growing clades, however, A2/re contains no amino acid substitutions in the hemagglutinin (HA) segment. Moreover, hemagglutination inhibition assays did not suggest substantial antigenic differences between A2/re viruses and viruses sampled during the 2016-2017 season. Rather, we observe that the A2/re clade was the result of a reassortment event that occurred in late 2016 or early 2017 and involved the combination of the HA and PB1 segments of an A2 virus with neuraminidase (NA) and other segmentsa virus from the clade A1b. The success of this clade shows the need for antigenic analysis that targets NA in addition to HA. Our results illustrate the potential for non-HA drivers of viral success and necessitate the need for more thorough tracking of full viral genomes to better understand the dynamics of influenza epidemics

Formyl Peptide Receptor as a Novel Therapeutic Target for Anxiety-Related Disorders

Formyl peptide receptors (FPR) belong to a family of sensors of the immune system that detect microbe-associated molecules and inform various cellular and sensorial mechanisms to the presence of pathogens in the host. Here we demonstrate that Fpr2/3-deficient mice show a distinct profile of behaviour characterised by reduced anxiety in the marble burying and light-dark box paradigms, increased exploratory behaviour in an open-field, together with superior performance on a novel object recognition test. Pharmacological blockade with a formyl peptide receptor antagonist, Boc2, in wild type mice reproduced most of the behavioural changes observed in the Fpr2/3(-/-) mice, including a significant improvement in novel object discrimination and reduced anxiety in a light/dark shuttle test. These effects were associated with reduced FPR signalling in the gut as shown by the significant reduction in the levels of p-p38. Collectively, these findings suggest that homeostatic FPR signalling exerts a modulatory effect on anxiety-like behaviours. These findings thus suggest that therapies targeting FPRs may be a novel approach to ameliorate behavioural abnormalities present in neuropsychiatric disorders at the cognitive-emotional interface

Evidence of population bottleneck in Astragalus michauxii (Fabaceae), a narrow endemic of the southeastern United States

Genetic factors such as decreased genetic diversity and increased homozygosity can have detrimental effects on rare species, and may ultimately limit potential adaptation and exacerbate population declines. The Gulf and Atlantic Coastal Plain physiographic region has the second highest level of endemism in the continental USA, but habitat fragmentation and land use changes have resulted in catastrophic population declines for many species. Astragalus michauxii (Fabaceae) is an herbaceous plant endemic to the region that is considered vulnerable to extinction, with populations generally consisting of fewer than 20 individuals. We developed eight polymorphic microsatellites and genotyped 355 individuals from 24 populations. We characterized the population genetic diversity and structure, tested for evidence of past bottlenecks, and identified evidence of contemporary gene flow between populations. The mean ratios of the number of alleles to the allelic range (M ratio) across loci for A. michauxii populations were well below the threshold of 0.68 identified as indicative of a past genetic bottleneck. Genetic diversity estimates were similar across regions and populations, and comparable to other long-lived perennial species. Within-population genetic variation accounted for 92% of the total genetic variation found in the species. Finally, there is evidence for contemporary gene flow among the populations in North Carolina. Although genetic factors can threaten rare species, maintaining habitats through prescribed burning, in concert with other interventions such as population augmentation or (re)introduction, are likely most critical to the long term survival of A. michauxii.Keywords: Pinus palustris, Endemism, Microsatellites, Genetic diversity, Astragalus, Bottlenec

The Leucine Zipper Domains of the Transcription Factors GCN4 and c-Jun Have Ribonuclease Activity

Basic-region leucine zipper (bZIP) proteins are one of the largest transcription factor families that regulate a wide range of cellular functions. Owing to the stability of their coiled coil structure leucine zipper (LZ) domains of bZIP factors are widely employed as dimerization motifs in protein engineering studies. In the course of one such study, the X-ray structure of the retro-version of the LZ moiety of yeast transcriptional activator GCN4 suggested that this retro-LZ may have ribonuclease activity. Here we show that not only the retro-LZ but also the authentic LZ of GCN4 has weak but distinct ribonuclease activity. The observed cleavage of RNA is unspecific, it is not suppressed by the ribonuclease A inhibitor RNasin and involves the breakage of 3′,5′-phosphodiester bonds with formation of 2′,3′-cyclic phosphates as the final products as demonstrated by HPLC/electrospray ionization mass spectrometry. Several mutants of the GCN4 leucine zipper are catalytically inactive, providing important negative controls and unequivocally associating the enzymatic activity with the peptide under study. The leucine zipper moiety of the human factor c-Jun as well as the entire c-Jun protein are also shown to catalyze degradation of RNA. The presented data, which was obtained in the test-tube experiments, adds GCN4 and c-Jun to the pool of proteins with multiple functions (also known as moonlighting proteins). If expressed in vivo, the endoribonuclease activity of these bZIP-containing factors may represent a direct coupling between transcription activation and controlled RNA turnover. As an additional result of this work, the retro-leucine zipper of GCN4 can be added to the list of functional retro-peptides